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2345 samples were sent for evaluation of serum ANCA at Brigham and Women’s Hospital between January 2011 and May 2013. Of these, 1998 (85%) had a negative IF, 147 had an atypical IF, and 200 had a P-ANCA pattern, a C-ANCA pattern, or both. The IF and MPO/PR3 tests results for all samples are summarized in Table 1. mpo 5000 slot